How can I seperate HDL(high density lipoprotein) subclasses(like HDL 2a, 2b, 3a, 3b, 3c)by ultracentrifugation?

I seperated lipoproteins(HDL, LDL, VLDL) by ultracentrifugation but I can not progress. I have problem about g(RCF) and hour for ultracentrifuge to seperate HDL subclasses. I followed a article...

28 March 2017 4,490 7 View