I am Sorting T-cell subpopulations from PBMC, I just want to know weather using Fc-blocker is mandatory or we can avoid using that step?

I used to isolate CD8+ cells from PBMC using MACS sorting, then will proceed with FACS sorting of both CD28+CD27+ and CD28-CD27- cells (Double positive and double negative). Before adding the CD8+...

20 April 2023 3,730 3 View