Mengying Xia

2 Questions 8 Answers 0 Followers

Questions related from Mengying Xia

Does anyone know how to induce autophagy in primary cultured hippocampus neuron?

Can I just follow the regular protocol in cell line that use EBSS and stave the neuron for several time points? But I don't know whether this kind of harsh condition suitable for neuron? I have...

05 May 2015 1,020 8 View

Use CRISPR/Cas to make knock in transgenic mouse, how can I provide an HDR template?

In Rudolf Jaenisch's Cell 2013 Paper (One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering), they just mentioned that they injected 126bp...

04 April 2014 3,334 1 View