Mena Krishnan

3 Questions 4 Answers 0 Followers

Questions related from Mena Krishnan

How to identify the source of sporadic/random contamination in my cell culture flasks?

I grow LNCaP prostate cancer cells in T flasks (vented neck, filter cap) in RPMI 1640 medium supplemented with 10% FBS and 1% Pen-Strep. While subculturing the cells, I am observing a strange...

03 March 2018 3,199 1 View

Why do I always get a very low cell count when I count cancer cells using Haemocytometer?

I have counted different cell lines like HeLa, LNCap.FGC, PC-3, CHO-b using a haemocytometer on T-25 and T-75 flasks. But even when the flasks are fully confluent, or 90% confluent, I get a very...

09 September 2015 6,042 4 View

KB cell lines revived, attached and then refused to attach on splitting. Why?

I had got a vial of KB cell lines from abroad, preserved in dry ice. The packaging and transport were in proper form. I revived the cells in a T-75 flask and they started to attach after 10 days...

07 July 2015 4,418 4 View