How to identify the source of sporadic/random contamination in my cell culture flasks?

I grow LNCaP prostate cancer cells in T flasks (vented neck, filter cap) in RPMI 1640 medium supplemented with 10% FBS and 1% Pen-Strep. While subculturing the cells, I am observing a strange...

05 March 2018 3,573 1 View