How to optimize solubility of my heterologous protein overexpressed at 0.04mM of IPTG and 26C incubation ?

Good morning/afternoon I am over-expressing a bacterial protein (dimer) in BL21 E. coli, which it works even with 0.04mM of IPTG, incubated at 26C. However, when I want to check the solubility of...

21 August 2017 2,341 5 View