Melissa Yeow

4 Questions 8 Answers 0 Followers

Questions related from Melissa Yeow

Has anyone tried the EchoCLEAN TM CleanUp Kits from BioEcho Life Sciences for DNA cleanup? How is the efficiency like?

I've been having trouble with purifying my DNA post-PCR using gel extraction techniques as I lose all my DNA and have a 230nm peak which may be contamination by ethanol of guanidinium salts. Any...

12 December 2018 5,756 0 View

What is the best way to purify DNA during SOE PCR?

According to protocols I found from Nature (Heckman et al 2007) and Cold Springs Harbour (Forloni et al 2018), the PCR products must be purified using gel electrophoresis and then a QIAquick gel...

11 November 2018 7,607 10 View

Trouble purifying PCR product using Qiagen gel extraction kit and Monarch gel extraction kit, 230nm peak?

I start with impure PCR products of >500ng/ul, 260/280=1, but peaks present at 260, and end up with peaks near 230, about 10ng/ul concentrations of 260/280=1.5 or so, which I assume is not DNA...

11 November 2018 3,996 9 View

Which is the best tool for operon prediction in bacteria?

I'm looking at the gene sequences of a pair of enzymes and related processing proteins and am trying to figure out whether they are in the same operon, and where the operon begins and ends.

09 September 2018 8,617 8 View