Melissa M Torres

4 Questions 6 Answers 0 Followers

Questions related from Melissa M Torres

How long do human brain tissue samples last after protein isolation?

Hello! I am currently running a western blot using human AD tissue samples. I prepared these samples over a year and a half ago. I am having trouble standardizing these proteins with Beta Actin. I...

03 May 2024 9,542 3 View

How to get successful bacterial plasmid growth?

I am currently running a plasmid isolation. I am using a bacterial stab to grow the plasmids. We have used this stab a few times now. Unfortunately, we have not had the chance to make glycerol...

09 April 2024 2,006 4 View

How can I have a better transfection rate of 50% for my HT-22 hippocampal neuronal cells?

I am currently working with HT-22 cells by transfecting them with bacterial plasmids to perform IHC. However, we have not had much success with reaching a 50% transfection rate. I usually do about...

18 January 2024 7,757 6 View

How can I fix my transfected cells for immunofluorescence protocol?

Hello. I plan to do a simple immunofluorescence procedure with HT-22 cells. I plan to transfect the cells and fix them. I do not believe I will have time to come in on the weekend. Is it possible...

12 December 2023 6,621 2 View