Melissa C Edwards

8 Questions 9 Answers 0 Followers

Questions related from Melissa C Edwards

How do I know if I am injecting into the tail vein of a mouse correctly?

How do I know if I am injecting into the tail vein of a mouse correctly? what are some tips for a successful injection.

11 March 2022 1,944 3 View

Original stock tube of primer labelled with fam diluted with Milliq water has turned yellow?

Hi, I dilute my primers with Milliq water. My PCR was working fine for 5-6 runs but now it has stopped working. I tried going back to the original stocks in the -20 but the solution has turned...

19 March 2021 380 4 View

What is the best way to mix peptide concentrations before adding to cells for an ELISA?

Mixing peptide concentrations

18 August 2013 9,932 1 View

How do I calculate intervariability and intra-variability in a cell ELISA?

What should I be aiming for? Is the standard variability different to a normal ELISA?

17 August 2013 8,287 4 View

Is it necessary to adjust the pH when making PBS with calcium and magnesium?

Is it necessary to adjust the ph.

07 August 2013 6,550 9 View

If you incubate fibronectin for 2 hours RT compared to overnight 4 degrees does anyone know how much it changes the cells adhering to the plates?

If you put the plate in the fridge with the lid on is it still sterile? If not how do I make it sterile

31 July 2013 198 1 View

How to coat 96 well flat bottom plates at 5 ug/cm squared with 1 mg bovine fibronectin from Sigma?

How to work out which volume is needed to coat the wells? My colleague said I needed to add 3.300 ml of water and aliquot it in tubes at 100ul each tube. Then to make a working solution add 900 ul...

30 July 2013 7,082 7 View

Help with in cell ELISAs?

I am trying to develop an in cell ELISA to detect peptide binding to primary osteoarthritis fibroblast like synoviocytes. However I am having trouble with cells being dislodged throughout the...

28 July 2013 3,325 20 View