Melissa Chua

5 Questions 1 Answers 0 Followers

Questions related from Melissa Chua

Can we derive organoids from 2D cell culture?

I am keen to derive organoids from my existing primary tumour cell culture. Is there any existing protocol that you would recommend?

22 March 2018 837 3 View

Why does shRNA-mediated silencing have different effects on cell signalling as compared to that by siRNA-mediated silencing?

I started off by creating a stable knockdown model using a control shRNA and two shRNA specific for my gene-of-interest. I achieved 90% knockdown after antibiotic selection, and the knockdown...

29 August 2014 8,521 6 View

Can anyone help with loading controls for high molecular weight proteins?

My proteins of interests are ~350-500kDa. The usual loading controls have much lower molecular weights. I run 6% (non-gradient) gels. Are there any suitable loading controls out there?

03 February 2014 1,815 4 View

Why does radiotherapy (seemingly) induce greater amount of double strand breaks compared to chemotherapy?

There is very few current literature on how chemotherapy affects the DNA damage response and DNA repair, in the context of tumor resistance. Is there an explanation for this?

30 January 2014 1,562 2 View

Does lysing cells for too long harm phosphoproteins?

I lysed my cells in 1x Laemmli buffer for 2 hours by mistake (the usual time period is 15mins). Would this mistake degrade my samples? I'm looking for phosphoproteins.

28 November 2013 4,616 3 View