Melisa Ait Kaci

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Questions related from Melisa Ait Kaci

How can I get RAW264.7 cells to differentiate into osteoclasts?

I can't get RAW264.7 cells to differentiate into osteoclasts. I've tested different densities: 5000 cells/cm2 , 10,000 cells/cm² and 20,000 cells/cm² in 35mm polystyrene. I use DMEM medium, 10%...

13 June 2024 4,555 1 View

Is anyone experiencing the same problem with RAW264.7 cells?

Hello, I have been cultivating RAW264.7 cells from ATCC recently. I culture them in DMEM medium + 10% FBS at a density of 10,000 cells/cm² in a T75 flask with a volume of 10mL. For cell...

28 February 2024 4,429 2 View