Why am I losing all of my target protein in column wash during affinity chromatography purification ?

To purify the protein, I am using 0.5% Triton X-100 because it is hydrophobic. Lysis buffer has ne contain imidazole and elution buffer has 250 mM imidazole. I tried to optimize imidazole...

18 August 2022 9,451 8 View