Multiple stains on agarose gel after 16S rDNA amplification of milk microbiota with 27F-338R primers, even after optimisation. Is it normal?

I'm working on the 16S rDNA amplification of cow milk microbiota. For the last few months, I have been trying different primers and optimizing temperatures. It seems like I always have some smears...

28 July 2017 5,860 4 View