Melanie Vargas

4 Questions 9 Answers 0 Followers

Questions related from Melanie Vargas

Polymyxin B dose response with Pseudomonas-biofilm assay?

Hi all, I was hoping you could light me up on some results that I recently obtained with a biofilm assay. I am growing P.aeruginosa culture in a 96well plate to perform a simple biofilm assay with...

05 August 2016 6,543 4 View

Does anyone have experience with transduction assay using AAV ?

Hi there, I would need a bit of help for transduction assay using AAV. I've produced AAV using HEK293T cells (triple transfection) and would like to assess the transducing units number from my...

22 March 2016 7,372 10 View

Triple transfection mechanism : does GFP testifies of efficient transfection when expressed ?

Hi everyone, Pardon me if the question sounds a bit stupid but I just would like to be sure to understand the triple transfection mechanism. I'm performing triple transfection for AAV2 production...

24 September 2015 2,885 12 View

PI staining protocol for dead cells ?

Hi, I am trying to perform a very simple PI staining assay but still I have been struggling to settle my protocol. I was hoping you could help me. I want to perform FACS analysis on...

01 January 1970 5,186 6 View