What is the effect of seeding density on MCF-10A? What is the density and passage interval you work with, and at what passage number?

We have strange things going on in our MCF-10A flask. I am wondering if the seeding density could be the cause. We seed them at 3000-4000 cells/cm2 and passage them 2 times a week. But 1). The...

19 April 2014 2,674 1 View