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Can anyone suggest me about the flourcent primers for Aflp analysis?. FAM is the common flourcent dye which is used in one of the selective primers of the AfLp, I want to lable my ECOR1 primer...
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Greetings I am doing Aflp, for my pre selective amplification step i am using two primers Mse1+1 base and Ecor1+1 base. But both the priners have different annealing temp 52 and 55. I tried...
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