Mehdi Heidari Horestani

2 Questions 2 Answers 0 Followers

Questions related from Mehdi Heidari Horestani

How is it possible to have in RIN results, extra peaks in addition to normal peaks for common RNA markers?

I performed RNA extraction and by Agilent machine I got the RNA quality check ( RINe). In result graph, I have more than 3 sharp peaks (5s, 18 and 28s rRNA peaks) and one more that I do not have...

05 January 2023 9,983 0 View

What could be the reason(s) of not possible to see the knock down effect in protein level?

Hi everyone, I have done gene knock down by shRNA and also siRNA. After transfection, in RNA level I have effiecient (80%) knock down but it is not possible to see KD in protein level by western...

11 December 2022 8,750 5 View