Mehdi Ghaffarisharaf

3 Questions 5 Answers 0 Followers

Questions related from Mehdi Ghaffarisharaf

How to design experiment for 2D-DIGE with only 2 type of samples?

I want to design an experiment for 2D-DIGE and I have two types of samples, one: control tissue from healthy individuals and other from patients. besides comparing proteom of patients to control...

03 November 2016 3,598 2 View

Can cyanogen bromide cleavage be used in 2D-electrophoresis analysis?

I'm trying to do 2d analysis of some fibrillar protein aggregates, anyone knows if I can use cyanogen bromide cleavage in 2d analysis. This cleavage is going to generate some peptide fragments...

28 June 2016 5,696 2 View

Why 2D-DIGE labeling doesn't work?

I have been trying to do 2D-DIGE. I'm using Lumiprobe 3Dye™ 2D DIGE dyes for labeling, however after IEF and 2nd dimension, I found no spots on the gel. I tried to do the silver staining of the...

15 June 2016 1,447 7 View