Mehak Kaushal

4 Questions 3 Answers 0 Followers

Questions related from Mehak Kaushal

Can any one tell how we can purchase the following Novozyme enzymes ?

I could not find the product no in the sigma catalogue for the two novozyme enzymes under the trademarks CELLIC CTEC3™ and CELLIC HTEC3™. Following are the application of the 2 enzymes which have...

16 December 2016 9,259 8 View

Trying to amplify a gene from genomic DNA with primers with low GC content and attached restriction sites, what would be the optimum PCR conditions?

Forward – 5’GAC GGA TCC ACG CAT TAA AGT ATA TTG TGT 3’ (Tm- 66.2 °C) 30bp (with Bamh1 site) Reverse - 5’ GCC TCT AGA AAT TAA TGA GCG CTT TAA ACT 3’ (Tm- 65.2 °C) 30 bp (with Xba1 site) My gene...

22 April 2014 3,091 5 View

What would be the key difficulties found in expressing a membrane protein from gram negative bacteria in gram positive bacteria?

What would be the key points to be addressed while expressing a membrane protein from gram negative(E.coli) in gram positive clostridium acetobutylicum?

07 February 2014 4,152 0 View

Where can we purchase E.coli strain ER2275 (Methylation strain) used for methylation of E. coli-clostridium shuttle vector?

Can anyone tell me from where we can purchase E. coli strain ER2275? We tried from NEB but they have stopped supplying it. Also can someone tell me the equivalent strain with the same genotype?...

28 November 2013 9,316 4 View