Meghraj Chavhan

3 Questions 9 Answers 0 Followers

Questions related from Meghraj Chavhan

CRISPR-Cas9 (how to design a guide RNA) ?

What are the basic to design a sgRNA, designing tools, synthesis tools, target predictions, off target and on target efficacy, etc. A good application based review would be appreciated.

24 January 2023 6,560 3 View

QTL mapping does not necessarily require RILs at F6 or F7 generations; rather, F4 (or even F3) populations would be almost as useful as F6 or F7, Why?

I want to know how the power of F3 is comparable to that of F6 and F7 and why we use F3 generation for QTL mapping and not F6. What could be the possible outcome if had gone for mapping of same...

28 February 2022 880 4 View

I have been reading a lot about PCR and still I am ending confused. I need to know the difference between RT-PCR, real-time PCR, qPCR, RT-qPCR ?

I Know that RT-PCR is Reverse Transcription PCR when the starting material is RNA. Going for reverse transcription first (RNA to cDNA) and then it could be conventional PCR with the need for gel...

12 February 2021 6,970 4 View