Megha Tawate

2 Questions 1 Answers 0 Followers

Questions related from Megha Tawate

Can anyone provide a protocol for mitochondria isolation for western blot ?

Currently I am trying abcam protocol for it.i checked the cell lysis by trypan blue stain which is more than 90 %. but I am facing following issues 1. unable to remove beta actin in mitochondrial...

11 March 2024 7,411 1 View

Has anyone tried clonogenic assay for Sk Hep1 cell line? I am getting very low efficiency in control itself, hence can't proceed with the experiment?

I have tried clonogenic assay for Sk Hep1 cell line 4-5 times. But the clonogenic efficiency in control in itself is less than 10%. I have tried seeding up to 1000-3000 cells in 6 cm as well as...

22 November 2021 8,482 2 View