Megha Gupta

5 Questions 5 Answers 0 Followers

Questions related from Megha Gupta

What are key points when performing Arabidopis leaf tissue staining with FM4-64 dye?

I want to study endocytic pathway for a specific plant protein using FM4-64 dye and leaf tissue from A. thaliana transgenic plants. Most of the studies were performed using only root tissues. Have...

02 May 2023 5,361 0 View

Unable to amplify AtFLS2 CDS from Arabidopsis Col-0 genotype?

I am trying to amplify FLS2 CDS from Col-0 genotype. Primers are correct and also tried gradient PCR with different type of high fidelity Taq polymerase. But no success yet. Any suggestions? Do I...

20 November 2022 3,231 5 View

Problem in localization of test gene along with GFP in yeast cells?

Hi there, I am trying to localize one of my plant test gene in yeast cells. I m using pDR196 yeast expression vector. I cloned GFP and test gene under PMA promoter (Yeast specific promoter). I...

17 July 2018 2,007 4 View

Hi there, Suitable vectors for plant tissue culture o model legume Medicago truncatula?

Hi, I am trying to generate some overexpression lines of Medicago truncatula. I have to go through plant tissue culture and agrobacterium mediated transformation. Earlier I have used...

13 June 2018 1,004 9 View

Hi there, how to dissolve of chitin powder from shrimp cells?

Hi there, I am doing one experiment in which I have to apply chitin powder from shrimp cells exogenously on my plant samples. Can anyone please suggest how to dissolve this chemical? There are...

08 June 2018 3,125 3 View