Megan Schupp

2 Questions 1 Answers 0 Followers

Questions related from Megan Schupp

IHC with PBST (Triton-x) instead of PBS. Are my samples ruined?

I have been doing immunohistochemistry on brainslices. However, I used 1 x PBST instead of PBS. Should i keep going with this protocol? Or start again?

02 October 2024 2,683 2 View

Best puromycin concentration for US02 transfection?

I am using the US02 cell line to perform an siRNA knockdown with an lipofection-based method (lipofection 3000). It has a puromycin selection marker. However, I am not sure what the best working...

30 September 2024 9,797 2 View