Meenakshi Sudhakaran

2 Questions 5 Answers 0 Followers

Questions related from Meenakshi Sudhakaran

Has anyone had any issues with THP1 macrophage polarization flow cytometry analysis?

I treat THP1 cells with 100 ng/ml PMA for 48 hrs and then stimulate them with 20 ng/ml of IL-4 and IL-13 for 48hrs (M2) and with 20 ng/ml IFN-gamma and 10 ng/ml LPS for 48hrs (M1). Then I leave...

07 June 2019 684 5 View

Can anyone suggest a protocol to detach THP-1 differentiated macrophages from tissue culture plates?

Hi, I am working with macrophage-cancer cell co-cultures. I have been trying to detach THP-1 differentiated macrophages (grown in RPMI-5% FBS) from tissue culture plates for FACS analysis. But I...

31 January 2019 5,502 6 View