Md.Rezwanul Haque

2 Questions 4 Answers 0 Followers

Questions related from Md.Rezwanul Haque

How can I avoid protein precipitation?

I need suggestion about my current problem. I purified serine hydroxymethyltransferase protein using Ni-NTA resin and enough amounts and purity of protein was observed on SDS-PAGE. But the protein...

09 September 2017 9,312 6 View

Hi , I am working with serine hydroxymethyl transferase. can anyone tell me which lysis buffer is better for purification of this protein?

For purification of this protein i used lysis buffer( 20mM tri s HCl pH=8,with 0.5M Nacl and 0.2M imidazol), (20mM NaPB pH=6.5,with 0.5M Nacl and and 0.2M imidazol), (1x PBSl pH=7,and 0.5M...

05 May 2017 6,526 2 View