Md Saiful Islam

5 Questions 19 Answers 0 Followers

Questions related from Md Saiful Islam

Is it necessary to measure the protein concentrations during EROD assay with fish liver microsome?

I found in some literature that they measure the total protein concentration according to the Bradford method. But I dont know why it is necessary. Thanks in advance.

01 January 1970 3,333 2 View

Is there any fixed range for Michaelis-Menten constant Km ?

I am now checking the PAPP substrate with PP2A at pH 8.4.

01 January 1970 2,472 5 View

In EROD assay is it mandatory to isolate the microsomal part for fluorometric determination ?

What kind of problem may face without isolation ? Usually after homogenization the liver, the microsomal part is separated and used for EROD assay?

01 January 1970 9,816 3 View

Why NADPH is added to the microsomal solution to initiate the Enzymatic reaction in EROD assay? What is the role of NADPH here?

Thank you for your kind consideration.

01 January 1970 3,230 2 View

How to calculate the surface area of the attached gold mesh electrode?

I am using this gold mesh as working electrode in the electrochemical cell. Now it is required to calculate the surface area. Thanks in advance.

01 January 1970 309 1 View