Md Reyazul Islam

5 Questions 6 Answers 0 Followers

Questions related from Md Reyazul Islam

What is the minimum concentration of glycoproteins (in total) required for analysis of N-Glycans using MALDI-TOF mass spectrometry?

I have expressed a human glycoprotein in plant ER through a signal sequence and it also contains ER retention signal at the C-terminus. Thus, if this protein contains an N-glycan moiety, it should...

12 December 2018 9,443 3 View

Should I change my PCR condition or design longer primer for low GC content (32% GC)?

I am trying to amplify a gene from plasmid DNA which has A-T rich sequence and low GC content, 32% GC. However, I could get the desired product which is around 900bp, but, I found lots of PCR...

10 October 2015 6,129 5 View

What is the best stripping solution recipe (in house) and method for PVDF membrane?

What is the best stripping solution recipe (in house) and method for PVDF membrane? A) Do you agree this is the best stripping solution recipe? ** 200mM Glycine, pH 2.3 ( 1 hour incubation and...

09 September 2015 4,950 1 View

How can I optimize PCR conditions for a reverse primer of length 51 base pairs (Tm 82 Deg) and forward Primer of length 24 base pairs (Tm 72.5 Deg)?

Hello I am trying to amplify a plasmid DNA using a forward primer of 24 base pairs and reverse of 51 base pairs. In the reverse Primer among 51bp, 24 is hybridizing sequence and remaining 27bp is...

06 June 2015 5,370 7 View

How to overcome the elution of non-specific bands in protein purification by Ni-NTA resin under native condition (with 6XHis-tag)?

I have eluted my protein with 250mM imidazole and got some non specific bands in the elution and yield also not good. Besides, during washing step i was used 20 mM imidazole washing buffer and...

02 February 2014 9,553 12 View