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I have expressed a human glycoprotein in plant ER through a signal sequence and it also contains ER retention signal at the C-terminus. Thus, if this protein contains an N-glycan moiety, it should...
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I am trying to amplify a gene from plasmid DNA which has A-T rich sequence and low GC content, 32% GC. However, I could get the desired product which is around 900bp, but, I found lots of PCR...
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What is the best stripping solution recipe (in house) and method for PVDF membrane? A) Do you agree this is the best stripping solution recipe? ** 200mM Glycine, pH 2.3 ( 1 hour incubation and...
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Hello I am trying to amplify a plasmid DNA using a forward primer of 24 base pairs and reverse of 51 base pairs. In the reverse Primer among 51bp, 24 is hybridizing sequence and remaining 27bp is...
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I have eluted my protein with 250mM imidazole and got some non specific bands in the elution and yield also not good. Besides, during washing step i was used 20 mM imidazole washing buffer and...
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