Md. Atiqur Rahman

5 Questions 22 Answers 0 Followers

Questions related from Md. Atiqur Rahman

Can anyone suggest the best way to transfect cloned plasmid vector into a mammalian cell line?

Hi everyone!I want to transfect my cloned plasmid vector into thyroid cancer cell lines (8505C, B-CPAP, K-1, MB-1 and BHT-101). Can anyone suggest me the best way to do the transfection and advise...

10 October 2014 9,884 8 View

How to re-probe dry PVDF western blot membrane?

I want to re-probe my previously detected western blot PVDF membrane. PVDF membrane is now in a dry and good condition. Could you give me ideas how can I do that?

10 October 2014 9,732 15 View

How to do western blot for phosphorylated protein?

I want to do western blot for phosphorylated serine/threonine kinase protein. How can I prevent the target phosphorylated protein from dephosphorylation during protein extraction and doing western...

02 February 2014 4,551 4 View

How to clone a 59 bp shRNA into a 6990 bp vector? What are the things I need to be careful about to get a successful clone?

Currently I am cloning a 59 bp shRNA into a 6990 bp vector. The shRNA contains 5' phosphate. I did a double-digestion of the vector with Xho I and Hind III. Then I run a gel to confirm that the...

02 February 2014 5,401 22 View

How to separate fibroblast cells from carcinoma cells in primary cell culture?

Currently I'm doing primary cell culture of carcinoma by ex-plant method. In the culture, I observed fibroblast cells along with carcinoma cells and fibroblast cells are growing faster than the...

01 January 2014 4,536 15 View