Md. Monirul Islam

5 Questions 7 Answers 0 Followers

Questions related from Md. Monirul Islam

Is the growth mediums for HUVEC and HPAEC cells are same?

I want to start HUVEC and HPAEC cell culture. Both are endothelial cell.But, My question is , is it require different types of mediums or the EGM-2 is OK for both?

03 March 2019 6,592 2 View

How to prepare absolute RNA wash solution ?

I am isolating RNA from whole blood for micro-array purpose. For this, I have to perform DNase treatment for purification of collected RNA. There are some commercially available reagent named...

10 October 2018 2,784 5 View

Is TE buffer 8.00 instead of TE 7.50 is OK for primer dilution?

I need to dilute GAPDH primer for PCR. In the brochure they mentioned TE buffer pH 7.5 as the diluent. But, I have TE buffer 8.00 at my hand now. Is it OK or may cause any deviation with TE 8.00?

10 October 2018 4,394 9 View

How to get a good control value(PBS) lesser than unknown sample in ELISA for MPO-DNA?

I am using ELISA method for detecting MPO-DNA from blood plasma for my current research purpose. I use 25 micro liter plasma sample and 75 micro liter PBS in each well of microplate as unknown and...

09 September 2017 983 3 View

Can I use Ammonium Sulfate for SDS-PAGE gel preparation instead of Ammonium persulfate (APS)?

I want to make SDS-PAGE gel for westernblot. But I have no APS at my hand now. What will be the alternative of APS?

07 July 2017 322 8 View