Md. Enamul Haque

5 Questions 6 Answers 0 Followers

Questions related from Md. Enamul Haque

Having blue smear during M13 phage titering, any idea?

I am using M13 phage peptide library for biapanning of specific peptide. After first 2 rounds of screening, i am now observing a blue layer on agar plate. I have diluted the phage upto highest...

09 September 2019 6,237 3 View

Can anyone suggest me effective concentration of Etoposide for A549 lung carcinoma cells and TRAIL concentration for MDA MB 231 cell apoptosis

Hi, i am trying to induce apoptosis in A549 cells and MDA MB 231 cells. I use etoposide for A549 cells and TRAIL for MDA MB 231 cells. I usually detect apoptosis induction by Annexin V staining...

04 April 2016 2,529 2 View

How can I make calcium free DMEM cell culture media?

I want to observe my cells protein expression in calcium free condition. I am culturing the cells in normal DMEM media. Does anybody have the experience that show how I can make that media ca free...

01 January 2015 9,001 7 View

Why is DAPI unable to penetrate the cell membrane but Hoechst stain does?

DAPI is usually used to stain fixed/permeabilized cells whereas Hoechst stain can be used to stain live cells also.

01 January 2014 2,286 6 View

Why do phage capsid express more no proteins by one single insert gene?

Usually as i know, 1 gene express one protein. Then how phage capsid express more of no proteins by one gene such as P III or PVIII ?

08 August 2013 2,539 9 View