Md. Shahinozzaman

7 Questions 11 Answers 0 Followers

Questions related from Md. Shahinozzaman

How to search AIST Database with chemical shifts?

Can anyone tell me how to search AIST database with proton/carbon NMR chemical shifts? Before I tried, but in the long run failed to get the suggested chemical structure. Your cooperation would...

30 October 2017 9,359 3 View

Which method should I follow next to purify the active natural products?

I have collected one active fraction from Si-gel CC using Hexane:EtOAc gradient. The active fraction eluted with 3:2 ratio. Now its time to purify the active compounds from this fraction. TLC...

22 September 2017 1,808 6 View

How to elucidate the structure using NMR chemical shifts?

I would like to elucidate chemical structure of an unknown sample using proton-NMR, Carbon-NMR, 2D-NMR and Mass spectrum Data. Can anybody suggest me how to do this and what type of references I...

20 September 2017 450 9 View

How to purify Nicotinic acid derivatives from the reaction mixture?

I am using Nicotinic acid for synthesizing some ester derivatives. I am using EDC/DMAP/DMF. Whenever I tried to purify the ester from reaction mixer with EtOAc or EtOAc-Hexane solvents...

14 August 2017 2,995 7 View

How to isolate specific peak (which represent bioactive compound) using preperative HPLC?

I would like to isolate more polar compound from the extract. In this regard, I am supposed to use HPLC for successful isolation of the compound (s). Can you please suggest me the procedure for...

15 August 2016 1,748 4 View

How to reduce DMSO concentration in cell culture based assay?

I use different cancer cells to expound Bioactivites of the isolated/target compounds. I dissolve the compounds in 100% DMSO, and I also use 24-well plate. In each well, I put 1 mL cell...

29 July 2016 1,239 1 View

What would be the HPLC conditions if I want to check the saponin content?

I have prepared different extracts of sea cucumber sample using different organic solvents. Can anyone suggest me the overall conditions of HPLC (mobile phases, time, column etc.) I need to to...

22 July 2016 3,556 2 View