Md Atikur Rahman

4 Questions 6 Answers 0 Followers

Questions related from Md Atikur Rahman

Which one is more preferable for characterization of a novel protein - Deletion or overexpression?

Generally, people can use either deletion or overexpression strategy to do characterization of any novel protein candidate. Can anyone describe or suggest any reference paper discussing the pros...

22 January 2022 9,153 4 View

How to study of novel proteins in bacterial host?

Hi, I have validated some novel proteins in my targeted bacteria. Now, I want to study the effects of these candidates by overexpressing them inside the bacterium. I have targeted three phenotypic...

01 December 2021 6,666 1 View

How to do homology analysis of a new small ORF in bacteria?

I have validated the expression of some putative small ORF in my target bacteria. I want to do the homology analysis of those small ORF candidates. Ans, I also want to check the -10 and -30 boxes,...

24 November 2021 5,943 1 View

Can anyone help me to solve my struggle of getting PCR products?

I am using Q5® High-Fidelity DNA Polymerase reaction system. I am following the standard protocol. First, I tried with 1-degree celsius less than the recommended Tm value from the NEB Tm...

29 March 2021 1,777 8 View