Md. Ajijur Rahman

3 Questions 4 Answers 0 Followers

Questions related from Md. Ajijur Rahman

Why does the culture of Ganoderma on PDA preserved in refrigerator not growing?

We grew a wild variety of Ganoderma by tissue culture on PDA. The pure culture of the isolate was preserved in a 4C refrigerator. Surprisingly, after two weeks of preservation when we tried to...

13 November 2023 2,537 1 View

Can a gene be lost after preservation at -80C in glycerol?

I isolated some species of bacteria from oral cavity containing a gene (secret). I detected the gene by PCR in the genomic DNA obtained from broth cultures. I stored the broth cultures in 20%v/v...

07 September 2015 3,373 5 View

Can anyone suggest a good protocol for the preparation of B. subtilis competent cells?

I tried the protocol described by Hardy (1985) but failed. So, I do need a protocol which could solve my problem.

14 October 2014 7,965 3 View