Does fixation with 4% PFA in 1X PBS for o/n at 4 degrees destroy GFP during intracellular immunostaining on cover slips?

HEK 293 cells were transfected with GFP tagged plasmid in earlier before doing immunostaining on cover slips. Under flurescence microscope I saw GFP continuously. But, during intracellular...

01 January 1970 2,913 8 View