Md. Monirul Islam

6 Questions 5 Answers 0 Followers

Questions related from Md. Monirul Islam

How to decrease losing of cells during collection and washing steps of Neutrophils to measure ROS?

I want to measure neutrophils derived intracellular ROS using DCF-DA as fluorescent probe by mirco-plate reader. As neutrophils are suspension cells, they normally do not attach with culture...

12 July 2019 3,408 4 View

Is the growth mediums for HUVEC and HPAEC cells are same?

I want to start HUVEC and HPAEC cell culture. Both are endothelial cell.But, My question is , is it require different types of mediums or the EGM-2 is OK for both?

28 March 2019 390 2 View

Why fluorometric ROS measurement by DCF-DA is inconsistent?

I am trying to measure PMN intra-cellular ROS using DCF-DA by microplate reader. Sometimes, the fluorescence intensity of blank (only meida+DCF-DA) is higher than that of sample readings. Can...

14 February 2019 7,465 6 View

How to get a good control value(PBS) lesser than unknown sample in ELISA for MPO-DNA?

I am using ELISA method for detecting MPO-DNA from blood plasma for my current research purpose. I use 25 micro liter plasma sample and 75 micro liter PBS in each well of microplate as unknown and...

11 September 2017 8,337 3 View

Endothelial Permeability using FITC-Albumin

I am using FITC-Albumin from Sigma. The stock solution is 1mg/ml.The pore size of the insert is 0.45 micron. Now, my question is, how much amount of FITC-Albumin soln. should be passed through the...

01 January 1970 4,186 0 View

Need to Inhibit inter-cellular VEGF protein

I am working with HUVEC and HPAEC cells. I need to block cell released inter-cellular VEGF proteins so that, it can not bind to VEGF receptor. Can anybody suggest, any primary VEGF anti-body (with...

01 January 1970 3,164 1 View