Md Mahmodul Hasan Sohel

4 Questions 17 Answers 0 Followers

Questions related from Md Mahmodul Hasan Sohel

What are the challenges you faced during working with extracellular miRNAs?

Dear friends and fellows, It will be great to know your experiences working with extracellular/circulating or exosomal miRNAs. We are facing different problems starting from sample isolation to...

01 February 2016 8,816 2 View

How can I incorporate SD value with Fold change for qRT-PCR data?

Daer all, currently I am calculating some of my real time PCR data using Excel 2007. When I incorporate SD value directly with higher fold change values (>5) the bar apparently seems OK....

16 June 2015 1,944 14 View

Can anyone suggest a RBC lysis buffer recipe?

Dear all, I am working with bovine granulosa cells. Recently, I have moved to a new lab and I lost my previous protocol to prepare RBC lysis buffer which is suitable for granulosa cells collected...

04 February 2015 7,397 9 View

Is there any method to estimate miRNA concentration from raw ct values?

I am trying to to interpret miRNA expression in certain sample. I have only raw ct value. It would be very nice to include concentration in relation to ct value.. has anyone done it??? help and...

04 March 2014 4,989 3 View