Why it is recommended to use primers that are of length between 18 - 30 base pair for sequencing? Why longer primers fails to execute the sequencing?

I have been sequencing PCR products in ABI Prism310 for more than two years and most of the sequencing attempts were successful while I used primers of 18-25 bases long. However, now I am working...

19 July 2018 1,501 9 View