Md Imran

2 Questions 3 Answers 0 Followers

Questions related from Md Imran

How an appropriate taxa could be assign to the CAZyme genes identified in a metagenome?

We have isolated metagenome from seawater. The whole metagenome was sequenced using NGS platform. The sequencing reads were assembled and whole metageomic sequence obtained. Genes were predicted...

11 May 2018 4,006 4 View

Are there any modified methods to isolate the high molecular weight (~15kb) recombinant plasmid from E.coli strain JM109?

I was trying to clone a gene into EcoRI site of pUC-18 by shotgun cloning method. A fragment of approx. 15 kb gets cloned and now I am trying to isolate the recombinant plasmid. However, every...

30 May 2013 6,020 7 View