Md Hassanur Rahman

2 Questions 10 Answers 0 Followers

Questions related from Md Hassanur Rahman

What are the causes of failed DNA sequencing reaction?

I cloned 1.6 kb gene into pTAC2 expression vector (linear : 2786 bp). I performed a diagnostic restriction digestion to confirm the insert. I used Bigdye terminal v3.1/1.1 cycle sequencing kit and...

21 April 2017 2,564 11 View

Why do we use 50 mM NaCl and 1 mM EDTA during making 10 X annealing buffer ?

I want to know the role of NaCl and EDTA in buffer for annealing oligonucleotide.

01 December 2016 8,978 3 View