What will be the PCR annealing temperature of SREBP2 primer?

SREBP2 primer seq is Forward 5'-GGAGACCATGGAGACCCTCAC-3' (GC%-62, Tm-65.3) reaction conc 10pmol/ul Reverse 3'-AGACAATGGGACCTGGCTGAA-5' (GC% 52, Tm-61.3) reaction conc 10pmol/ul I used several...

08 June 2022 787 3 View