Mayur Mukut Murlidhar Sharma

3 Questions 2 Answers 0 Followers

Questions related from Mayur Mukut Murlidhar Sharma

How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

07 February 2021 521 3 View

What can be the problem in the isolation of Plasmid?

I've tried transformation of a 1.7 kb gene with DH5 alpha strain of competent cells and also used PuC19 as a control. After selecting colonies and broth-culturing overnight, I isolated plasmid....

28 December 2020 3,751 5 View

How can i reduce primer dimers and get my cDNA PCR amplification done?

I am performing High-fidelity general PCR using Takara PrimeSTAR™ HS DNA Polymerase , but there is no amplification and very dense bands of primer dimers are visible. the cDNA is of superior...

25 March 2020 1,711 3 View