Maykel López

2 Questions 2 Answers 0 Followers

Questions related from Maykel López

Why do I get a thick milky-whitish upper layer after sonication and further centrifugation from HepG2 cells lysates?

Hi folks, I am running a ChIP protocol on HepG2 cells and I got a thick milky-whitish upper layer after sonication and further centrifugation (there are 3 phases, the upper milky that I mentioned,...

22 December 2015 2,169 2 View

Does anyone have a protocol to transfect HepaRG cells?

I am planing to use HepaRG cells as a glucose metabolism model. But I need to siRNA and plasmid transfect the cells in order to get my model working. Does any one have a protocol for HepaRG...

13 June 2014 7,569 1 View