May Meltzer

6 Questions 9 Answers 0 Followers

Questions related from May Meltzer

How do you choose the detergent concentration for protein purification?

hey i am using 2%OG and 1%DDM what concentration do i need to use with DM? is there a formula to calculate? Why usualy using 1%DDM and 2% OG (what % is higher then the CMC?) thanks

01 January 2019 900 9 View

What are the plats for eby100- yeast cells with PYES2 plasmid?

hey i have EBY100- Saccharomyces cerevisiae yeast cells. with the plasmid PYES2 (the map is added) How do i make selective plats for it? what is the protocol? and what possitive and what negative...

11 November 2017 8,803 0 View

Where can i get information about PITY and PYES2 plasmids?

hey all i have inserted a gene into PITY and PYES2 in yeast(why both i will explain) the PITY is an integral plasmid (goes in the genome of the yeast)and the PYES2 isn't. i am looking to built...

11 November 2017 4,304 0 View

How to built Yeast library with mutations only in 2 helixes?

hey all thank you for your help in advance i want to built Yeast library with mutations only in 2 helixes. so i have plasmid. in it my gene. now lets say i want to do PCR but enter modifications...

07 July 2017 7,717 3 View

How can i produce genomic analysis (DNA extract) with very low concentration of DNA from Yeast?

hey all i need to extract DNA from yeast cells. but how can i do it from one yeast or very low number of yeast cells. is there a special protocol for it? Thanks May

06 June 2017 4,284 10 View

Is there plasmid that will have gpcr+sequence that will lead him to the membrane of a yeast?

i have Yeast i need GPCR on the yeast membrane how do i do it? i need help to understand which plasmid can work for me. how do i plan in and make it work. it should have GFP on it so i can see...

04 April 2017 6,420 0 View