May Hijazi

2 Questions 5 Answers 0 Followers

Questions related from May Hijazi

How can I optimize the experiment to be able to see the nuclear protein fused with YFP in the nucleus?

I am over-expressing a protein of interest (implicated in DNA repair) in fusion with yfp in Nicotiana Benthamiana leaves. The proteomics data showed that the protein is cytoplasmic as well as...

21 October 2014 4,728 3 View

Decreasing protein viscosity without disrupting protein-DNA complex

I am trying to purify my protein (protein implicated in the DNA recombination process) which was overexpressed in benthamiana plants using the FPLC system, so, I grind my infiltrated leaves and...

05 March 2013 4,551 8 View