How can you use the fluorescent intensity of mCherry to calculate BiFC efficiency from split mVenus if the two proteins have different brightness?

I am starting a bimolecular fluorescence complementation (BiFC) assay. In the same vector, there will be 'protein A' (A) fused to 1/2 mVenus in one cassette and in another cassette there will be...

18 July 2020 1,375 1 View