Maximilian Paradiz

5 Questions 8 Answers 0 Followers

Questions related from Maximilian Paradiz

What method can I use for purification of a His-tagged synthetic peptide?

We want to order a 20-mer synthetic peptide. We are considering adding a His-tag to the 20-mer (making it a 26-mer) and ordering the crude product, and then purifying it ourselves using his-tag...

06 June 2014 4,757 5 View

Any suggestions for displaying BLAST sequence alignment results in PyMol?

In order to identify the sequences that are likely to be important for an enzyme's function I perform a BLAST search and then align the sequences of several orthologs which are 40%-99% similar....

12 December 2013 3,181 3 View

How do you identify a specific enzyme in a library of mutants?

Lets say I want to make an enzyme that catalyzes the conversion of A into B, where neither A nor B affect the cell's viability in any significant way. I know that there is an enzyme "X" which...

08 August 2013 4,954 3 View

Is there a known genetic mutation/mechanism through which a given polypeptide can have its amino acid sequence inverted pre-translationally?

For example: A DNA sequence is translated into the sequence: N-MKTSTRFLDGYFPVAANK-C -Mutation- Which leads to the production of: N-(Met)KNAAVPFYGDLFRTSTKM-C The obvious problem is...

05 May 2013 6,905 2 View

How do protein structure representations affect the way we think about proteins?

In 1981 Jane S. Richardson published "The Anatomy and Taxonomy of Protein Structure", which strongly influenced the scientific community to agree on a general way to represent protein structures -...

05 May 2013 885 2 View