Maxim Geeroms

4 Questions 8 Answers 0 Followers

Questions related from Maxim Geeroms

Is it normal for ASC to become bigger after the first passage?

Dear all, When I culture the stromal vascular fraction (SVF) from murine inguinal adipose tissue, the size of the cells increase. I see this when passaging my cells the first time and the...

10 October 2016 8,004 8 View

Does culturing of ASC lead to a 100% pure collection of ASC? Or does it contain other adherent cell types?

Dear all, I am starting an experiment with adipose derived stem cells (ASC), but I would like some help or confirmation for the following issue. Background: I am harvesting murine fat from the...

07 July 2016 9,635 18 View

T test vs Wilcoxon-Mann-Whitney test vs Fisher-Pitman permutation test after RT-PCR comparative CT experiments (ΔΔCT method)?

Hello, I have done RT-PCR in which I compare the expression of a gene of interest (TGF-beta1) in a control group with 4 samples and a treatment group with 4 samples. I calculated delta CT for the...

04 April 2016 7,434 3 View

What is the best method to perform Ki-67 and C/EBPα staining on frozen adipose tissue sections?

I am looking for an optimal method to perform a Ki67 and C/EBPα staining on frozen sections from adipose tissue. My current protocol exists of the following steps and gives disappointing...

02 February 2016 4,276 2 View