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I ´purify GST-tagged transcription factors from E.coli (BL21 RIPL). I either lyse my cells with ultrasound (sonicator) or with pressure (french press). Sometimes I encounter a turbidity in my...
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I am expressing transcription factors in E.coli. Sometimes after the lysis (Lysosome and ultrasound) I see transparent gel like matter in my lysate which can't be cleared by centrifugation (18 000...
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