Max Schnepf

2 Questions 5 Answers 0 Followers

Questions related from Max Schnepf

What can a turbidity in a bacterial lysate be?

I ´purify GST-tagged transcription factors from E.coli (BL21 RIPL). I either lyse my cells with ultrasound (sonicator) or with pressure (french press). Sometimes I encounter a turbidity in my...

05 December 2016 8,854 7 View

How to avoid genomic DNA(?) aggregation in bacterial lysis?

I am expressing transcription factors in E.coli. Sometimes after the lysis (Lysosome and ultrasound) I see transparent gel like matter in my lysate which can't be cleared by centrifugation (18 000...

11 August 2016 3,284 3 View