Max Mc Cluskey

3 Questions 4 Answers 0 Followers

Questions related from Max Mc Cluskey

What are the necessary parameters for measuring GAPDH NADH production over time in a UV transparent 96-well plate?

I am looking to measure NADH production at 340 nm by GAPDH (from crude protein extract) over time in a UV transparent 96-well plate. So far I am unable to detect any signal and I was wondering if...

13 February 2020 7,476 2 View

How could one measure enzymatic activity of the whole glycolytic pathway in a time-course manner?

I am looking to measure enzymatic activity of the glycolytic pathway by measuring ATP production over time. Most ready made kits on the market provide single time point measurments and do not...

28 November 2019 9,496 5 View

Do Michaelis-Menten principles apply if i measure kinetic rates for a chain of enzymes (glycolysis for example)?

I am measuring the production of NADH in a cell extract provided with glucose. If I measure the formation of a NADH over time, which is produced by the combined action of a chain of different...

01 January 1970 7,852 4 View