Hi everyone, I have performed RT-PCR with cDNA recently, but may I know why I got this kind of amplification plot even for the housekeeping gene??

For your information, I am using ABI StepOne Plus machine. The housekeeping gene is Beta-actin. However, the results showed its melting curve and fluorescence plot look good. Appreciate it if...

14 November 2022 9,848 1 View